Oxidation of Intermediates of the Tricarboxylic Acid Cycle by Extracts of Azotobacter Agile.
نویسندگان
چکیده
منابع مشابه
Tricarboxylic Acid Cycle in Pseudomonas Aeruginosa”
During the past few years scattered information has appeared which indicates that at least some bacteria probably possess a tricarboxylic acid cycle. Lockwood and Stodola found that Pseudomonas jluorescens formed considerable quantities of a-ketoglutaric acid when growing on a glucose medium under conditions of intense aeration (1). Ajl and Werkman found evidence for at least parts of the conve...
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During the investigation of the Krebs cycle in Azotobacter agile, strain 4.4 (Repaske and Wilson, 1953), it was found that whole cells normally required 20 minutes before adaptation to succinate, but succinoxidase activity could be demonstrated in cell-free extracts made from unadapted cells. If extracts prepared in a 10 kc Raytheon sonic oscillator were centrifuged at 145,000 G for 30 minutes,...
متن کاملThe oxidation of acetate by extracts of Micrococcus lysodeikticus.
Evidence has been presented (Saz and Krampitz, 1954) which demonstrates that a-ketoglutarate and citrate are intermediates on the pathway of acetate oxidation by partially lysed cell preparations of Micrococcus lysodeikticu8. Results reported in this publication extend and further substantiate our previous findings. Despite the fact that condensing enzyme can be demonstrated in many aerobic bac...
متن کاملTricarboxylic acid cycle in Pseudomonas aeruginosa.
During the past few years scattered information has appeared which indicates that at least some bacteria probably possess a tricarboxylic acid cycle. Lockwood and Stodola found that Pseudomonas jluorescens formed considerable quantities of a-ketoglutaric acid when growing on a glucose medium under conditions of intense aeration (1). Ajl and Werkman found evidence for at least parts of the conve...
متن کاملEvidence for the occurrence of Permeases for tricarboxylic acid cycle intermediates in Pseudomonas aeruginosa.
Washed suspensions and cell-free extracts of Pseudomonas aeruginosa grown on Lemco agar, to which intermediates of the tricarboxylic acid cycle had been added, were tested for their ability to oxidize succinate, fumarate, malate, pyruvate, acetate, a-oxoglutarate and citrate. Whole organisms had lag periods of 2-3 hr. before citrate was oxidized rapidly, except when citrate or acetate had been ...
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ورودعنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 39 4 شماره
صفحات -
تاریخ انتشار 1953